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      <image:caption>Figure 5 The titers of pre-existing antibodies are not affected by deamidation. Anti-AAV9 antibodies were detected in 16 human serum samples. WT or deamidated AAV9 capsids were coated passively onto a plate, serum samples were then added after blocking, and pre-existing antibodies were detected using a colorimetric reaction involving anti-human FC-HRP antibodies and TMB substrate. A titer was calculated by subtracting the signal from the sham ELISA and then interpolating the point where there is no longer change in OD signal in the reciprocal dilutions. (A) Anti-AAV titer against WT or deamidated (mutated) capsids in 16 healthy individuals. (B) Data were divided into 3 groups based on titer against WT capsids, then were compared to titer against mutated capsids. WT&lt;De indicates that the titer against deamidated (mutated) capsids is more than 20% higher than the titer against WT capsids. WT&lt;De indicates that the titer against deamidated (mutated) capsids is more than 20% lower than titer against WT capsids.</image:caption>
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